MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

Figure 2 Upregulation of miRNA-584-5P inhibits the osteogenic differentiation of periosteal stem cells induced by hypoxia. A: MiRNA-584-5p (miR584-5p) expression in stem cells; B: Alkaline phosphatase activity; C: Intracellular calcium ion level; D: Alizarin red S staining chelated Ca²⁺ form an orange-red complex; E: Osteogenic differentiation-related protein expression. One-way ANOVA and t tests were used to demonstrate differences between the miR584-5p mimic and negative control groups. ^aP < 0.05; ^bP < 0.01. ALP: Alkaline phosphatase; RUNX2: RUNX family transcription factor 2; BMP2: Bone morphogenetic protein 2; HIF-1α: Hypoxia-inducible factor 1; miRNA: MicroRNA; NC: Negative control.