Adipose tissue in bone regeneration - stem cell source and beyond

doi:10.4252/wjsc.v14.i6.372

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jun 26, 2022; 14(6): 372-392
Published online Jun 26, 2022. doi: 10.4252/wjsc.v14.i6.372

Table 1 Factors that influence adipose-derived mesenchymal stem cells secretome content and release[99-101]

Donor	Tissue	Culture conditions	Cell manipulation
Species	Type (sWAT, vWAT)	2D vs 3D (spheroid culture, cell sheets), suspension culture	Preconditioning (IFN-γ, TNF-α, LPS)
Age	Anatomic location (abdominal, brachial, mammary)	Hypoxia	Differentiation (osteogenic, adipogenic)
BMD (obesity)	Method of procurement (lipectomy, liposuction)	Plating density, co-culture	Physical factors (electromagnetic fields, pulsed electromagnetic fields)
Disease (T2D, metabolic syndrome, lipodystrophy)	Methods of tissue processing, enzymatic digestion, mechanical trituration	Media formulation (serum containing versus serum free, growth factor addition)

T2D: Type 2 diabetes mellitus; sWAT: Subcutaneous white adipose tissue; vWAT: Visceral white adipose tissue; IFN-γ: Interferon gamma; TNF-α: Tumor necrosis factor alpha; LPS: Lipopolysaccharides; BMD: Bone mineral density.

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Citation: Labusca L. Adipose tissue in bone regeneration - stem cell source and beyond. World J Stem Cells 2022; 14(6): 372-392
URL: https://www.wjgnet.com/1948-0210/full/v14/i6/372.htm
DOI: https://dx.doi.org/10.4252/wjsc.v14.i6.372

Labusca L. Adipose tissue in bone regeneration - stem cell source and beyond. World J Stem Cells 2022; 14(6): 372-392 [PMID: 35949397 DOI: 10.4252/wjsc.v14.i6.372]

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