Optimization of adipose tissue-derived mesenchymal stromal cells transplantation for bone marrow repopulation following irradiation

Figure 5 Characterization of repopulated bone marrow post-whole-body irradiation in the normal control, irradiated, and stem cell-treated groups. A-I: The expression levels of Sca-1, c-Kit, CD34, interleukin (IL)-3R, IL-7R, CD45RA, CD80, and CD206 in the bone marrow (BM) at 3 wk after post-whole-body irradiation (WBI) was examined using flow cytometry; J: mRNA expression in the BM at weeks 1, 2, and 3 post-WBI was evaluated using reverse transcription PCR. ^aP < 0.05 vs controls, as indicated. NC: Normal control; ST: Stem cell-treated; RT: Irradiated; Sca-1: Stem cells antigen-1; c-KIT: Tyrosine-protein kinase KIT; CD: Cluster of differentiation; IL: Interleukin; TNF-α: Tumor necrotic factor-a; M-CSF: Macrophage colony-stimulating factor; GM-CSF: Granulocyte-macrophage colony-stimulating factor; SCF: Stem cell factor; SDF-1: Stromal cell-derived factor 1; TPO: Thrombopoietic growth factor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.