Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors, and their in vivo implantation regenerated the intervertebral disc degeneration

Figure 6 Stemness of transfected human umbilical cord mesenchymal stem cells. A: After 21 d of in vitro culture in normal and chondro-induction media, transfected cells were immunocytochemically stained for the expression of mesenchymal stem cell (MSC) stemness marker Stro-1. Alexa fluor 546 labeled phalloidin was used to visualize F-actin of the cellular cytoskeleton. The nuclei were stained with DAPI; B: The fluorescent intensity of Stro-1 was quantified and showed the expression of Stro-1 protein. ^cP < 0.001 vs MSCs. iCPCs: Induced chondro-progenitor cells.