SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4

Figure 7 SPOC domain-containing protein 1 promotes the proliferation of spermatogonial stem cells via adenylate kinase 4. A and B: Western blotting shows the level of adenylate kinase 4 (AK4) after re-expression in SPOC domain-containing protein 1 (SPOCD1) knockdown spermatogonial stem cells (SSCs); C: Cell Counting Kit-8 was used to detect cell proliferation after AK4 was overexpressed in SPOCD1-knockdown SSCs; D and E: Cellular DNA synthesis was detected by 5-ethynyl-2’-deoxyuridine (EdU) analysis; F and G: Western blotting shows the protein levels of promyelocytic leukemia zinc finger (PLZF) and proliferating cell nuclear antigen (PCNA) after transfection with AK4 expression plasmid in SPOCD1 inhibited SSC; H and I: Cell apoptosis analysis after re-expression of AK4 using flow cytometry. Scale bar in D: 20 μm. ^aP < 0.05.