Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation

Figure 2 Etanercept cytotoxicity analysis and human adipose-derived stem cells chondrogenic differentiation ability identification. A: The growth curves of human adipose-derived stem cells (hADSCs) treated with chondrogenic medium containing etanercept were measured using the CCK-8 method; B: Scratch test performed to determine the migration of hADSCs treated with chondrogenic medium containing etanercept; C and D: Toluidine blue staining and Western blot detection of chondrogenic differentiation marker proteins. ^aP < 0.05. GM: Human adipose-derived stem cells (hADSCs) grown in growth medium alone; CHE: hADSCs treated with chondrogenic differentiation medium and 1 μg/mL etanercept.