Copyright
©The Author(s) 2022.
World J Stem Cells. Nov 26, 2022; 14(11): 777-797
Published online Nov 26, 2022. doi: 10.4252/wjsc.v14.i11.777
Published online Nov 26, 2022. doi: 10.4252/wjsc.v14.i11.777
Table 1 Culture conditions of limbal stem cells
| Culture condition | Canine amniotic membrane | Atelocollagen gel | Temperature-responsive culture dish |
| Culture | |||
| Adhesion time, d | 1 | 1 | 2 |
| Convergence time, d | 5 | 7 | 14 |
| Layers | 6-8 | 4-5 | 2-3 |
| Immunofluorescence | |||
| Ki-67 | Basal layer + | Basal layer ++ | Basal layer - |
| K3 | Each layer + | Each layer except basal layer + | Each layer + |
| ABCG2 | Only in basal layer + | ||
| P63 | Basal layer + | 2-3 layers, including the basal layer + | Basal layer + |
- Citation: Ying PX, Fu M, Huang C, Li ZH, Mao QY, Fu S, Jia XH, Cao YC, Hong LB, Cai LY, Guo X, Liu RB, Meng FK, Yi GG. Profile of biological characterizations and clinical application of corneal stem/progenitor cells. World J Stem Cells 2022; 14(11): 777-797
- URL: https://www.wjgnet.com/1948-0210/full/v14/i11/777.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v14.i11.777