Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway

Figure 6 Senescence-related and SIRT1/PGC-1α pathway-related mRNA and proteins assay. A: The expression of senescence-related proteins (P16 and P21) in different groups; B-C: Quantitative analysis of P16 and P21 protein expression results; D-E: Quantitative analysis of P16 and P21 mRNA expression results; F-G: Quantitative analysis SIRT1/PGC-1α pathway-related mRNA. All data are expressed as the mean ± SD. ^aP < 0.05, ^bP < 0.01 compared with control group; ^cP < 0.05, ^dP < 0.01 compared with H₂O₂ group; ^eP < 0.05, ^fP < 0.01 compared with H₂O₂ + UA group. SIRT1/PGC-1α: Silent information regulator of transcription 1/PPAR gamma coactivator-1α; UA: Urolithin A.