Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway

Figure 4 Senescence-associated β-Galactosidase staining assay and cell cycle assay. A: Senescence-associated β-Galactosidase (SA-β-Gal) staining results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) in different groups. Senescent cells exhibit blue-stained high expression of SA-β-Gal. (scale bar = 25 μm); B: Quantitative analysis of SA-β-Gal staining results; C: Cell cycle results of NPMSCs in different groups; D: Quantitative analysis of cell cycle results. All data are expressed as the mean ± SD. ^aP < 0.05, ^bP < 0.01 compared with control group; ^cP < 0.05, ^dP < 0.01 compared with H₂O₂ group; ^eP < 0.05, ^fP < 0.01 compared with H₂O₂ + UA group. SA-β-Gal: Senescence-associated β-Galactosidase; NPMSCs: Nucleus pulposus-derived mesenchymal stem cells; UA: Urolithin A.