Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway

Figure 3 Cell viability assay, cell proliferation assay and cytotoxicity assay. A: Cell counting kit-8 (CCK-8) results of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) treated with different concentrations and times of urolithin A (UA); B: CCK-8 results of NPMSCs treated with different concentrations and times of SR-18292; C: EdU assay results of NPMSCs in different groups. Green fluorescence represents cells in a proliferating state, and blue fluorescence represents cell nucleus (scale bar = 25 μm); D: Quantitative analysis of EdU results; E: Cytotoxicity results of NPMSCs treated with H₂O₂, H₂O₂ + UA and H₂O₂ + UA + SR-18292. All data are expressed as the mean ± SD. ^aP < 0.05, ^bP < 0.01 compared with control group; ^cP < 0.05, ^dP < 0.01 compared with H₂O₂ group; ^eP < 0.05, ^fP < 0.01 compared with H₂O₂ + UA group. CCK-8: Cell counting kit-8; NPMSCs: Nucleus pulposus-derived mesenchymal stem cells; UA: Urolithin A.