SmartFlareTM is a reliable method for assessing mRNA expression in single neural stem cells

Figure 1 SmartFlare^TM detection in 3 d in vitro neural stem cells. A and B: The expression pattern for both SmartFlare^TM CD133 and Oct4 probes showed a diffuse and spotted-like fluorescence from the perinuclear area to the peripheral cytological processes; C: SmartFlare^TM probe for Actin showed a robust and overall localization of red fluorescence as indicative of positive mRNA expression; D: Scramble probe-treated cells were almost completely lacking in unspecific red background. Nuclei were stained by Hoechst dye. Scale bar: 50 μm.