Nanofat: A therapeutic paradigm in regenerative medicine

Table 1 Modified nanofats

Type	Total volume of lipoaspirate	Procedure
Supercharged nanofat[24]	L + 80 mL	(1) First 80 mL lipoaspirate – Automatic filtration (120 μm filter) and centrifugation at 1300 rpm for 10 min in a closed system with 20 mL Luer lock syringes. The lower SVF was collected and further filtered to obtain the final 20 mL product; (2) Second 80 mL – Emulsification (30 passages) into two 10 mL Leur lock syringes; and (3) The SVF suspension obtained from the first lipoaspirate fraction should be mixed with the emulsified fat to form a supercharged nanofat
Evo modified nanofat[24]	L	(1) Slow centrifugation at 80 rpm for 3 min; and (2) Homogenization of emulsified fat through Luer lock system (20 passages)
Centrifuged modified nanofat[24]	L	(1) Direct centrifugation at 1300 rpm for 10 min; and (2) After discarding SVF, the concentrated aspirate of centrifuged fat should be emulsified by 30 passages through the Luer lock system

SVF: Stromal vascular fraction.