©The Author(s) 2021.
World J Stem Cells. Oct 26, 2021; 13(10): 1394-1416
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1394
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1394
Table 2 Alternative splicing analysis tools based on isoform-based method and count-based method (including the exon- and event-based approaches)
| Tools | Human/ plants/ animals | Types of detecting splices | Type of tool | Year of publish | Ref. | Citation |
| Isoform-based method | ||||||
| Cuffdiff2 | All | - | Linux | 2012 | [109] | 9550 |
| HMMSplicer | All | - | Linux | 2010 | [110] | 79 |
| PennDiff | All | - | Linux | 2018 | [111] | 9 |
| rSeqDiff | All | - | R | 2013 | [112] | 29 |
| DiffSplice | All | - | Windows/Linux | 2013 | [113] | 141 |
| NSMAP | All | - | MatLab package | 2011 | [114] | 46 |
| MISO | All | SE / A3SS / A5SS / MXE / TandemUTR / RI / AF / AL | Linux | 2010 | [115] | 1166 |
| Exon-based method | ||||||
| TopHaT | All | - | Linux | 2009 | [116] | 11026 |
| DEXSeq | All | - | R | 2012 | [117] | 1091 |
| edgeR | All | - | R | 2010 | [118] | 19012 |
| JunctionSeq | All | - | R | 2016 | [119] | 81 |
| limma | All | - | R | 2015 | [120] | 11033 |
| rSeqNP | All | - | R | 2015 | [121] | 10 |
| Event-based method | ||||||
| MAJIQ | All | SE / A5SS / A3SS | Linux | 2016 | [122] | 171 |
| rMATS | All | SE / A5SS / A3SS / RI / MXE | Linux | 2014 | [123] | 746 |
| SUPPA | All | SE / A5SS / A3SS / RI / MXE / AF / AL | Linux | 2015 | [124] | 134 |
| SUPPA2 | All | SE / A5SS / A3SS / RI / MXE / AF / AL | Linux | 2018 | [125] | - |
| ASGAL | All | SE / A5SS / A3SS / RI | Linux | 2018 | [126] | 12 |
| Astalavista version 3.0 | All | SE / A5SS / A3SS / RI | Linux | 2012 | [127] | 62 |
| LeafCutter | All | SE / A5SS / A3SS | Linux | 2018 | [128] | 200 |
| SpliceGrapher | All | SE / A5SS / A3SS / RI | Linux | 2012 | [129] | 132 |
| KisSplice | All | SNPs, indels and AS events (SE / A5SS / A3SS / RI ) | Linux | 2012 | [130] | - |
| Alt Event Finder | All | SE | Linux | 2012 | [131] | 24 |
| Matt | All | SE / A5SS / A3SS / RI | Linux | 2018 | [132] | 14 |
| ALEXA-seq | All | SE / A5SS / A3SS / RI | Linux | 2010 | [133] | 340 |
| Outrigger | All | SE / A5SS / A3SS / RI | Linux | 2016 | [134] | 86 |
| ASDT | All | SE / A5SS / A3SS / RI | Perl | 2018 | [135] | 5 |
| SplicePie | All | SE / RI | R | 2015 | [136] | 17 |
| VAST-TOOLS | All | SE / A5SS / A3SS / RI | R | 2017 | [137] | 118 |
| spliceR | All | SE / A5SS / A3SS / RI / AF/AL | R | 2014 | [138] | 80 |
| Pro-Splicer | Human | SE / A5SS / A3SS | Website (http://prosplicer.mbc.nctu.edu.tw/) | 2003 | [139] | 50 |
| ASPicDB | Human | SE / A5SS / A3SS / RI / AF/AL | Website (http://srv00.recas.ba.infn.it/ASPicDB/) | 2006 | [140] | 33 |
| H-DBAS | Human | SE / A5SS / A3SS / RI / AF/AL | Website (http://www.h-invitational.jp/h-dbas/) | 2007 | [141] | 56 |
| ASIP | Plant | SE / A5SS / A3SS / RI | Website (http://www.plantgdb.org/ASIP/) | 2006 | [142] | 535 |
| ASG | Human | SE / A5SS / A3SS / RI | Website (https://brcwebportal.cos.ncsu.edu/asg/) | 2004 | [143] | 116 |
| VastDB | vertebrate | SE / A5SS / A3SS / RI | Website (https://vastdb.crg.eu/wiki/Main_Page) | 2017 | [137] | 118 |
| Astalavista | Human/ Animals | SE / A5SS / A3SS / RI | Website (http://astalavista.sammeth.net/) | 2007 | [144] | 205 |
| SpliceDitector | Plant/ Human | SE / A5SS / A3SS / RI | Windows | 2018 | [11] | 1 |
| dSpliceType | All | SE / A5SS / A3SS / RI / MXE | Windows/ Linux | 2015 | [145] | 4 |
| AltAnalyze | All | SE / A5SS / A3SS / RI / MXE | Windows/ Linux | 2010 | [146] | 253 |
- Citation: Ebrahimie E, Rahimirad S, Tahsili M, Mohammadi-Dehcheshmeh M. Alternative RNA splicing in stem cells and cancer stem cells: Importance of transcript-based expression analysis. World J Stem Cells 2021; 13(10): 1394-1416
- URL: https://www.wjgnet.com/1948-0210/full/v13/i10/1394.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v13.i10.1394