Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Figure 4 Anti- receptor for advanced glycated end products monoclonal antibody inhibits receptor for advanced glycated end products and human leukocyte antigen-DR expression. Lean adipose-derived stem cells (ASC) were co-cultured with mononuclear cells (MNC) at the 1:5 ASC to MNC cell ratio, in the presence of 1% glycated human serum albumin for 48 h, phytohemagglutinin A was added at 5 µg/mL. Anti-receptor for advanced glycated end products (RAGE) monoclonal antibody was added at a concentration of 20 µg/mL. cluster of differentiation (CD) 41, CD62P, intercellular adhesion molecule 1, human leukocyte antigen-DR, and RAGE expression were stained with fluorescent-conjugated antibodies and analyzed by cytofluorometry, using the DIVA software. This experiment is representative of two experiments performed with two different ASC. ASC: Adipose-derived stem cells; MNC: Mononuclear cells; G-HSA: Glycated human serum albumin; PHA: Phytohemagglutinin A; RAGE: Receptor for advanced glycated end products; mAb: Monoclonal antibody; CD: Cluster of differentiation; ICAM-1: Intercellular adhesion molecule 1; HLA: Human leukocyte antigen; FITC: Fluorescein isothiocyanate; PE: Phycoerythrin; APC: Allophycocyanin.