Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

Figure 2 Isolation procedure of endothelial colony-forming cells from placental chorionic villi. A: Depiction of the isolation of chorionic villus endothelial colony-forming cells from chorionic-villus cells by enzymatic dissociation of chorionic villus tissue, followed by CD31 magnetic bead sorting and subsequent manual clonal isolation and expansion; B: Morphology of the colonies formed by CD31⁺-sorted cells. CD31 was expressed only by colonies that had a cobblestone-like morphology and not by ones that displayed spindle shaped morphology. Two representative colonies are shown for each morphology; C: Immunofluorescence staining of isolated colonies for expression of CD31 and CD144. Scale bar = 100 µm.