Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

Figure 1 Characterization and cellular composition of the human early gestation placenta. Cellular composition of the human early gestation placenta chorionic villi was characterized. A: Immunofluorescence staining of CD31, CD34, von Willebrand factor and CD146 showed positive cells, depicted with arrows, located in chorionic villi and organized into blood vessel structures; B, C: Flow cytometry analysis of the surface markers of single cells derived by enzymatic dissociation of the placental chorionic villi; each marker’s expression is quantitively presented (data were expressed as mean ± SD, n = 3); D: Flow cytometry analysis of the co-expression of typical endothelial cell marker CD31 and other surface markers. Scale bar = 100 µm.