Effects of normobaric cyclic hypoxia exposure on mesenchymal stem-cell differentiation–pilot study on bone parameters in elderly

doi:10.4252/wjsc.v12.i12.1667

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Dec 26, 2020 (publication date) through May 21, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Stem Cells. Dec 26, 2020; 12(12): 1667-1690
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1667

Table 1 Primer sequences and product sizes

Gene	Primer sequence (5’ —> 3’)	Product size (bp)
Runt-related transcription factor 2 (RUNX2)	TGGTTAATCTCCGCAGGTCAC; ACTGTGCTGAAGAGGCTGTTTG	143
Osterix (SP7)	AGCCAGAAGCTGTGAAACCTC; AGCTGCAAGCTCTCCATAACC	163
Collagen, type I, alpha 1 (COL1A1)	CGCTGGCCCCAAAGGATCTCCTG; GGGGTCCGGGAACACCTCGCTC	263
Integrin-binding sialoprotein (IBSP)	AGGGCAGTAGTGACTCATCCG; CGTCCTCTCCATAGCCCAGTGTTG	171
Osteocalcin (BGLAP)	CCATGAGAGCCCTCACACTCC; GGTCAGCCAACTCGTCACAGTC	258
Vascular endothelial growth factor A (VEGFA)	GCGAGCAGCGAAAGCGACAGG; CGTCAGCGCGACTGGTC	114
Peroxisome proliferator-activated receptor gamma 2 (PPARG2)	GCGATTCCTTCACTGATACACTG; GAGTGGGAGTGGTCTTCCATTAC	136
Fatty acid synthase (FASN)	AAGCTGAAGGACCTGTCTAGG; CGGAGTGAATCTGGGTTGATG	146
Adipose triglyceride lipase (ATGL) or patatin-like phospholipase domain-containing protein 2 (PNPLA2)	CCAACACCAGCATCCAGTTCA; ATCCCTGCTTGCACATCTCTC	102
Glycerol-3-Phosphate Dehydrogenase 1 (GPD1)	ATACAGCATCCTCCAGCACAAG; GGATGATTCTGCAGGCAGTG	120
Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)	CTGGTTGCCTGCATGAGTGTG; GGGCAAAGAGGCTGGTCTTCAC	173
Osteoprotegerin (OPG)	GGCGCTACCTTGAGATAGAGTTCTG; TGTTTTCTACAGGGTGCTTTAGATGAC	160
Receptor activator for nuclear factor kappa B ligand (RANKL)	CGTCGCCCTGTTCTTCTATTTC; AAATGCAGTGAGTGCCATCTTC	74
Low-density lipoprotein receptor related protein 5 (LRP5)	TACTGGACAGACTGGCAGACC; GTGTAGAAAGGCTCGCTTGG	209
Low-density lipoprotein receptor related protein 6 (LRP6)	TACTGGCCAAATGGACTGACT; TGTTGCAAGCCAAAATGGAGT	211
Dickkopf Wnt signaling pathway inhibitor 1 (DKK1)	ATGCGTCACGCTATGTGCT; GGAATACCCATCCAAGGTGCTA	144
Polymerase RNA II polypeptide A (POLR2A)	TTTTGGTGACGACTTGAACTGC; CCATCTTGTCCACCACCTCTTC	125

Citation: Camacho-Cardenosa M, Quesada-Gómez JM, Camacho-Cardenosa A, Leal A, Dorado G, Torrecillas-Baena B, Casado-Díaz A. Effects of normobaric cyclic hypoxia exposure on mesenchymal stem-cell differentiation–pilot study on bone parameters in elderly. World J Stem Cells 2020; 12(12): 1667-1690
URL: https://www.wjgnet.com/1948-0210/full/v12/i12/1667.htm
DOI: https://dx.doi.org/10.4252/wjsc.v12.i12.1667