Copyright
©The Author(s) 2020.
World J Stem Cells. Dec 26, 2020; 12(12): 1652-1666
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1652
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1652
Table 1 Gene primer sequences used in this study with their annealing temperatures and product sizes
| No. | Genes | Primer sequence (5’-3’) | Annealing temperatures (ºC) | Product sizes (bp) |
| 1 | Interleukin 1 beta (IL-1β) | F: TCATCTTTGAAGAAGAGCCCGT | 60 | 170 |
| R: GTTCTGTCCATTGAGGTGGAGA | ||||
| 2 | Interleukin 6 (IL-6) | F: GATGGATGCTTCCAAACTGGATA | 58 | 140 |
| R: TGAATGACTCTGGCTTTGTCTTT | ||||
| 3 | Transforming growth factor (TGF-β) | F: TCGCCAGTCCCCCGA | 60 | 101 |
| R: TCGGGCTCCGGGTCA | ||||
| 4 | Vascular endothelial growth factor (VEGF) | F: CCAATTGAGACCCTGGTGGA | 60 | 195 |
| R: TCCTATGTGCTGGCTTTGGT | ||||
| 5 | Tumor necrosis factor (TNF-α) | F: CCTCTTCTCATTCCTGCTCGT | 60 | 144 |
| R: GATCTGAGTGTGAGGGTCTGG | ||||
| 6 | Bcl-2 associated X (Bax) | F: CTTCTTCCGTGTGGCAGC | 58 | 172 |
| R: CAGACAAACAGCCGCTCC | ||||
| 7 | Apoptosis regulator (Bcl-2) | F: ACCCCTGGCATCTTCTCC | 59 | 172 |
| R: AGAAGTCATCCCCAGCCC | ||||
| 8 | Matrix metallopeptidase 9 (MMP-9) | F: TACCAGCTACTCGAACCAATCA | 59 | 187 |
| R: AAATAAAAGGGCCGGTAAGGTG |
- Citation: Aslam S, Khan I, Jameel F, Zaidi MB, Salim A. Umbilical cord-derived mesenchymal stem cells preconditioned with isorhamnetin: potential therapy for burn wounds. World J Stem Cells 2020; 12(12): 1652-1666
- URL: https://www.wjgnet.com/1948-0210/full/v12/i12/1652.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v12.i12.1652