Pericyte-like differentiation of human adipose-derived mesenchymal stem cells: An in vitro study

Figure 2 Neural/glial antigen 2 immunoreactivity (green fluorescence) evaluated after one day (left column, 1 d), three days (middle column, 3 d) and six days (right column, 6 d) of cell growth. First row: Human adipose-derived mesenchymal stem cells (hASCs) cultured in basal medium; Second row: hASCs in basal medium stimulated with transforming growth factor; Third row: hASCs cultured in pericyte medium without pericyte growth supplement; Fourth Row: hASCs cultured in complete pericyte medium; Fifth row: Human retinal pericyte cells cultured in complete pericyte medium (hRPCs). Photomicrographs show that clear neural/glial antigen 2 immunoreactivity is present only in hASCs pre-cultured in complete pericyte medium and human retinal pericyte cells (last two rows). The immunostaining detected at day 1 is similar to that observed at day 3 and 6. Very weak immunostaining is visible in the other three hASC groups (first three rows). Blue fluorescence indicates DAPI staining of cell nuclei. Scale bar: 50 μm.