Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach

Figure 1 Static suspension culture and 24-well Elplasia^TM micro-well culture with human induced pluripotent stem cells. A-C:Induced pluripotent stem cell (iPSC)-derived embryoid bodies (EBs) formed under a suspension system and cultured with mTeSR™, respectively, on day 1 (A), day 3 (B), and day 7 (C); D-L: iPSC-derived EBs formed with single hiPSCs in micro-space cell culture plates at different cell densities: D-F: 2.5 × 10⁵ cells/mL; G–I: 5.0 × 10⁵ cells/mL; and J–L: 1.0 × 10⁶ cells/mL, respectively, on day 1 (D, G, and J), day 3 (E, H, and K), and day 7 (F, I, and L). Scale bars: A, D, G, and J 100 μm; resident graphs, 250 μm.