Basic Study
Copyright ©The Author(s) 2020.
World J Stem Cells. Jan 26, 2020; 12(1): 35-54
Published online Jan 26, 2020. doi: 10.4252/wjsc.v12.i1.35
Table 1 PrimeTime quantitative polymerase chain reaction assays used for droplet digital polymerase chain reaction
Product of interest: GeneReference gene or gene of interestAssay identificationSize of complimentary DNA amplicon, base pairs
Actin, betaReferenceHs.PT.39a.22214847109
Beta-2-microglobulinReferenceHs.PT.58v.18759587142
Glucuronidase betaReferenceHs.PT.58v.27737538127
Glycreraldehyde-3-phosphate dehydrogenaseReferenceHs.PT.39a.22214836142
Hypoxanthine phosphoribosyltransferase 1ReferenceHs.PT.58v.45621572148
Peptidylprolyl isomerase AReferenceHs.PT.58v.38887593. g101
Actin, alpha 2, smooth muscle, aortaGene of interestHs.PT.56a.2542642131
ATP binding cassette subfamily B member 5Gene of interestHs.PT.58.38502346106
ATP-binding cassette, sub-family G member 2Gene of interestHs.PT.58.20889058114
Collagen type I, alpha 1 chainGene of interestHs.PT.58.15517795130
Collagen type I, alpha 2 chainGene of interestHs.PT.58.26714160108
Integrin, subunit beta 1Gene of interestHs.PT.58.39883300141
Keratin 3Gene of interestHs.PT.58.25330614126
KeratocanGene of interestHs.PT.58.45594658130
Laminin, subunit alpha 1Gene of interestHs.PT.58.3170022129
Notch1Gene of interestHs.PT.58.23074795112
Proliferating cell nuclear antigenGene of interestHs.PT.58.476161194
Tumour protein p63, transcript variant 1, C-terminal isoform αGene of interestHs.PT.58.39019253108
Tumour protein p63, transcript variant 1, N-terminal isoformGene of interestHs.PT.58.19710922104
VimentinGene of interestHs.PT.58.38906895141