©The Author(s) 2020.
World J Stem Cells. Jan 26, 2020; 12(1): 35-54
Published online Jan 26, 2020. doi: 10.4252/wjsc.v12.i1.35
Published online Jan 26, 2020. doi: 10.4252/wjsc.v12.i1.35
Table 1 PrimeTime quantitative polymerase chain reaction assays used for droplet digital polymerase chain reaction
| Product of interest: Gene | Reference gene or gene of interest | Assay identification | Size of complimentary DNA amplicon, base pairs |
| Actin, beta | Reference | Hs.PT.39a.22214847 | 109 |
| Beta-2-microglobulin | Reference | Hs.PT.58v.18759587 | 142 |
| Glucuronidase beta | Reference | Hs.PT.58v.27737538 | 127 |
| Glycreraldehyde-3-phosphate dehydrogenase | Reference | Hs.PT.39a.22214836 | 142 |
| Hypoxanthine phosphoribosyltransferase 1 | Reference | Hs.PT.58v.45621572 | 148 |
| Peptidylprolyl isomerase A | Reference | Hs.PT.58v.38887593. g | 101 |
| Actin, alpha 2, smooth muscle, aorta | Gene of interest | Hs.PT.56a.2542642 | 131 |
| ATP binding cassette subfamily B member 5 | Gene of interest | Hs.PT.58.38502346 | 106 |
| ATP-binding cassette, sub-family G member 2 | Gene of interest | Hs.PT.58.20889058 | 114 |
| Collagen type I, alpha 1 chain | Gene of interest | Hs.PT.58.15517795 | 130 |
| Collagen type I, alpha 2 chain | Gene of interest | Hs.PT.58.26714160 | 108 |
| Integrin, subunit beta 1 | Gene of interest | Hs.PT.58.39883300 | 141 |
| Keratin 3 | Gene of interest | Hs.PT.58.25330614 | 126 |
| Keratocan | Gene of interest | Hs.PT.58.45594658 | 130 |
| Laminin, subunit alpha 1 | Gene of interest | Hs.PT.58.3170022 | 129 |
| Notch1 | Gene of interest | Hs.PT.58.23074795 | 112 |
| Proliferating cell nuclear antigen | Gene of interest | Hs.PT.58.4761611 | 94 |
| Tumour protein p63, transcript variant 1, C-terminal isoform α | Gene of interest | Hs.PT.58.39019253 | 108 |
| Tumour protein p63, transcript variant 1, N-terminal isoform | Gene of interest | Hs.PT.58.19710922 | 104 |
| Vimentin | Gene of interest | Hs.PT.58.38906895 | 141 |
- Citation: Wadhwa H, Ismail S, McGhee JJ, Van der Werf B, Sherwin T. Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy. World J Stem Cells 2020; 12(1): 35-54
- URL: https://www.wjgnet.com/1948-0210/full/v12/i1/35.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v12.i1.35