Derivation and applications of human hepatocyte-like cells

Table 2 Summary of transplantation studies using hepatocyte-like cells

Ref.	Animal model	Route	Proliferative stimulus	Type and number of cells	Donor / recipient (% engrafted)	Donor / recipient (% repopulated)	Time post-transplantation
Agarwal et al[24], 2008	NOD-SCID mice	Portal vein	CCl4-injured	106 hES-DEs	< 1%	NA	28 d
Basma et al[7], 2009	NOD-SCID mice	Spleen	Retrorsine and partial hepatectomy	1 × 106 hES-HLCs	NA	NA	21 d
Liu et al[77], 2011	NSG mice	Tail vein	dimethylnitrosamine -injured	0.1 - 2 × 106 hiPSC- multistage hepatic cells	2%–17%	8%–15%	56 d
Asgari et al[76], 2013	Normal mouse	Tail vein	CCl4-injured	1 × 106 hiPSC- HLCs	2 ± 0.7%	NA	35 d
Carpentier et al[73], 2014	MUP-uPA/ SCID/Bg mice	Spleen	NA	4 × 106 hiPSC-HLCs	1%-7%	< 1 to up to 20%	100 d
Song et al[20], 2015	Immunocompetent mice	Intraperitoneal cavity	NA	4.4×105 hiPSC-HLCs in capsules	NA	NA	24 d
Nagamoto et al[74], 2015	uPA/SCID mice	Spleen	NA	1 × 106 Ad-FNK-transduced hiPSC-HLCs	NA	NA	28 d
Nagamoto et al[78], 2016	Mice	hiPS-HLC sheet transplantation	2/3 partial hepatectomy and CCl4-injured	8 × 105 hiPSC-HLCs	NA	NA	14 d

DE: Definitive endoderm; HLCs: Hepatocyte-like cells; hPSCs: Human pluripotent stem cells.