Copyright
©The Author(s) 2019.
World J Stem Cells. Jun 26, 2019; 11(6): 347-374
Published online Jun 26, 2019. doi: 10.4252/wjsc.v11.i6.347
Published online Jun 26, 2019. doi: 10.4252/wjsc.v11.i6.347
Table 1 Antibodies used for immunofluorescence and for flow cytometry analysis
| Antigen | Company | Dilution | Incubation time | |
| Immunofluorescence staining | ||||
| CD73 | Thermo Fisher | Weston, Florida, USA | 1:100 | 1 h |
| CD90 | Abcam | Cambridge, UK | 1:100 | 1 h |
| CD105 | Dako | Palo Alto, California, USA | 1:10 | 1 h |
| CD34 | 1:100 | 30 min | ||
| CD45 | 1:100 | 30 min | ||
| CD56 | 1:80 | 30 min | ||
| CD146 | Santa Cruz | Dallas, Tx, USA | 1:25 | 1 h |
| PDGFRα | 1:25 | 1 h | ||
| PW1 | Atlas Antibodies | Bromma, Sweden | 1:100 | 1 h |
| Alexa Fluor | 1:700 | 40 min | ||
| 488 nm | ||||
| Donkey anti-mouse | ||||
| Alexa Fluor | Thermo Fisher | Weston, Florida, USA | 1:700 | 40 min |
| 488 nm | ||||
| Donkey anti-rabbit | ||||
| Alexa Fluor | 1:700 | 40 min | ||
| 594 nm | ||||
| Donkey anti-mouse | ||||
| Alexa Fluor | 1:700 | 40 min | ||
| 594 nm | ||||
| Donkey anti-rabbit | ||||
| Flow Cytometry | ||||
| CD73 (IgG) APC | BD Pharmingen | San Jose, California, USA | 1:25 | 30 min |
| CD90 (IgG) FITC | 1:25 | 30 min | ||
| CD105 (IgG) PE | 1:25 | 30 min | ||
| CD34 (IgG) FITC | 1:25 | 30 min | ||
| CD45 (IgG) APC | 1:25 | 30 min | ||
| CD56 (IgG) PE | 1:25 | 30 min | ||
| CD146 (IgG) PE | 1:25 | 30 min | ||
| Isotype controls: | 1:25 | 30 min | ||
| IgG APC | ||||
| IgG PE | ||||
| IgG FITC | ||||
- Citation: Kozlowska U, Krawczenko A, Futoma K, Jurek T, Rorat M, Patrzalek D, Klimczak A. Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues. World J Stem Cells 2019; 11(6): 347-374
- URL: https://www.wjgnet.com/1948-0210/full/v11/i6/347.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v11.i6.347