Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Figure 8 Evaluation of near-infrared fluorescence, bioluminescent images (in vivo) and magnetic resonance imaging signals (ex vivo) after 6 d of stroke induction. (I) Local blood perfusion images of the basal condition and 30 min after stroke induction by thermocoagulation; (II) graphic representation of local blood perfusion showing the decrease in perfusion from 80 to 20 au, comparing the basal condition to 30 min after stroke induction; (III) TTC staining of the sham and stroke groups performed 120 min after ischemic lesion induction; A, B: In vivo BLI and NIRF images of the sham_50 group; C: Ex vivo MRI results of the sham_50 group; D, E: In vivo BLI and NIRF images of the stroke_50 group; F, G: Ex vivo MRI results of the stroke_50 group (red arrows show the presence of nanoparticles, and a yellow arrow shows the stroke lesion); H: Graphic representation of quantification of the iron load via NIRF (red bars) and MRI (gray bars) in different groups in experiments 1 and 2, ^aP < 0.05, ^bP < 0.001, MRI vs NIRF; J: Graphic representation of BLI intensity (blue bars) in different groups in experiments 1 and 2, ^cP < 0.05, vs sham[50]-6 d (n = 7 rats/group). BLI: Bioluminescent images; NIRF: Near-infrared fluorescence;