Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review

doi:10.4252/wjsc.v11.i2.124

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Feb 26, 2019; 11(2): 124-146
Published online Feb 26, 2019. doi: 10.4252/wjsc.v11.i2.124

Table 2 Details of antibodies and incubation protocols used in the present study

Antibody	Isotype	Manufacturer	Dilution/incubation
runX2	Goat polyclonal	Santa Cruz Biotechnology (Dallas, TX, United States)	1:30, 4 °C, ON
Collagen type I	Rabbit monoclonal	Abcam (Cambridge, United Kingdom)	1:400, RT, 1 h
Alkaline phosphatase	Rabbit polyclonal	Quartett (Berlin, Germany)	Ready to use, 4 °C, ON
Von Willebrand factor	Rabbit polyclonal	Linaris (Dossenheim, Germany)	1:200, RT, 1h
CD146	Rabbit monoclonal	Abcam	1:50, RT, 1h
CD73	Mouse monoclonal	Antibodies-online (Atlanta, GA, United States)	1:100, 4 °C, ON
PPARγ	Mouse monoclonal	Santa Cruz Biotechnology	1:25, 4 °C, ON

ON: Overnight; RT: Room temperature.

Citation: Solakoglu Ö, Götz W, Kiessling MC, Alt C, Schmitz C, Alt EU. Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review. World J Stem Cells 2019; 11(2): 124-146
URL: https://www.wjgnet.com/1948-0210/full/v11/i2/124.htm
DOI: https://dx.doi.org/10.4252/wjsc.v11.i2.124