Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Table 1 Quantification of the multimodal nanoparticles-IR750 load and number internalized by human bone marrow mesenchymal stem cells_Luc via magnetic resonance imaging, inductively coupled plasma-mass spectrometry magnetic resonance imaging and near-infrared fluorescence

[Fe] in cell labeling (µg Fe/mL)	&Iron load /cell (pg Fe/mL)
	¥[Number MNP-IR750 per cell × 104]
	MRI	ICP-MS	NIRF
[5]	&0.94 ± 0.26	&1.32 ± 0.23	&2.12 ± 0.32
	¥1.19 ± 0.34	¥1.66 ± 0.28	¥2.64 ± 0.41
[10]	&2.54 ± 0.23	&2.73 ± 0.25	&3.02 ± 0.39
	¥3.20 ± 0.29	¥3.43 ± 0.31	¥3.77 ± 0.49
[20]	&4.71 ± 0.25	&4.82 ± 0.21	&5.14 ± 0.33
	¥5.92 ± 0.32	¥6.06 ± 0.26	¥6.41 ± 0.41
[30]	&5.31 ± 0.26	&6.34 ± 0.24	&6.52 ± 0.42
	¥6.66 ± 0.33	¥7.97 ± 0.30	¥8.17 ± 0.53
[40]	&6.20 ± 0.31	&6.83 ± 0.22	&7.12 ± 0.46
	¥7.79 ± 0.38	¥8.58 ± 0.27	¥8.79 ± 0.57
[50]	&6.30 ± 0.32	&6.71 ± 0.28	&7.3 ± 0.47
	¥7.92 ± 0.39	¥8.43 ± 0.35	¥9.17 ± 0.59
	Parameter fit curve
	mcell([Fe]) = mcellmax × (1-e-[Fe]/φ)
Parameter	MRI	ICP-MS	NIRF
mcellmax (pg Fe/mL)	7.91 ± 0.22	8.29 ± 0.49	7.93 ± 0.40
φ (µg Fe/mL)	14.64 ± 1.69	16.50 ± 3.37	18.90 ± 2.14

MRI: Magnetic resonance image; ICP-MS: Inductively coupled plasma - mass spectrometry; NIRF: Near-infrared fluorescence; m_cell([Fe]): MNP-IR750 load values internalized as a function of the labeling concentration; m_cell^max: maximum number of MNP-IR750 that could be internalized by hBM-MSC_Luc; φ: Constant equivalent of the adequate MNP-IR750 concentration for achieving 63% of m_cell^max after the cellular internalization process.