Developments in cell culture systems for human pluripotent stem cells

Table 2 Naïve pluripotent stem cell and extended pluripotent stem cell culture conditions

	Gafni et al[48]	Chan et al[49]	Valamehr et al[50]	Ware et al[51]	Theunissen et al[120]	Guo et al[47]	Ying et al[42]	Yang et al[15]	Yang et al[15]	Yang et al[14]
	Naive hESC	Naive hESC	Naive hESC	Naive hESC	Naive hESC	Naive hESC	Naive mESC	hEPSC	mEPSC	mEPSC
MEK inhibitor	PD0325901	PD0325901	PD0325901	PD0325901	PD0325901	PD0325901	PD0325901	-	-	PD0325901
GSK3 inhibitor	CHIR 99021	BIO	CHIR99021	CHIR99021	IM-12	CHIR99021	CHIR99021	CHIR99021	CHIR99021	CHIR99021
LIF	+	+	+	+	+	+	+	+	+	-
FGF2	+	+	+	+	-	-	-	-	-	-
TGFβ	+	+	-	Activin	Activin	-	-	-	-	-
ROCK Inhibitor	Y27632	Thiazovivin	Thiazovivin	Y27632	Y27632	Y27632	-	Y27632	-	-
Insulin	+	+	-	-	-	+	-	-	-	-
Other factors	JNKi; p38i; PKCi	Dorsomorphin	TGFi	TGFi; FGFRi; HDACi, STAT3i	B-RAFi; LCK/SRCi	PKCi	-	IWR1, dimethindene maleate, minocycline hydrochloride	Dimethindene maleate, minocycline hydrochloride	p38i, JNKi; SRCi; Tanki, WNTi
Base medium	KO DMEM	DMEM/F12	DMEM/F12	DMEM/F12	DMEM/F12/ Neurobasal	DMEM/F12/ Neurobasal	DMEM/F12	DMEM/F12/Neurobasal	DMEM/F12/Neurobasal	DMEM/F12
Medium Supplement	KOSR, NEAA, AlbuMAX I, L-glutamine, BME	TeSR1 components	KOSR, NEAA, BME, L-glutamine	GlutaMAX, KOSR, NEAA, BME, sodium pyruvate	B27/N2, L-glutamine, NEsAA, BME, BSA	N2/B27, L-glutamine, BME, Vc, selenium, putrescine, proges-terone	N2/B27	N2/B27, GlutaMAX, BME, BSA, KOSR	N2/B27, GlutaMAX, BME, BSA, KOSR	BME, GPS, NEAA, KOSR
Feeder	MEF optional	MEF	-	-	MEF	MEF	-	MEF	MEF	MEF
Coating	Gelatin/vitronectin	Matrigel	Matrigel/vitronectin	Matrigel	-	-	Gelatin	-	-	-
Markers	Surface markers[121]: CD7, CD77, CD75, CD130						Oct4, Nanog, Rex1	OCT4, KLF4, NANOG	Oct4, Nanog, Sox2, Sall4	Oct4, Nanog, Sox2, Klf4, Rex1, Esrrb
	Nuclear markers[119]: OCT4, NANOG, SOX2, KLF4,5,17, TFCP2L1, DPPA3,5

mESC: Mouse embryonic stem cell; hEPSC: Human extended pluripotent stem cell; mEPSC: Mouse extended pluripotent stem cell.