Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells

Figure 3 Functional characterization of acid extruders in the 5% CO₂/HCO₃^--buffered system. A-E: The trace shown in A showed a typical pH_i recovery slope after NH₄Cl prepulse-induced intracellular acidosis in HEPES-buffered solution as a control. The traces shown in B-E showed the effect of the removal of extracellular Na⁺ (Na⁺-free), addition of 90 μmol/L S0859 (S₉₀), addition of 30 μmol/L HOE 694 (H₃₀) and addition of S₉₀ + H₃₀ on the pH_i recovery slope; F: The curve of the pH_i recovery rates after the addition of Na⁺-free, S₉₀, H₃₀ and S₉₀ + H₃₀ were collected from 2-10 similar experiments shown in A-E. Error bars represent the mean ± SE.