Copyright
©The Author(s) 2018.
World J Stem Cells. Dec 26, 2018; 10(12): 196-211
Published online Dec 26, 2018. doi: 10.4252/wjsc.v10.i12.196
Published online Dec 26, 2018. doi: 10.4252/wjsc.v10.i12.196
Figure 2 Calibration of the BCECF fluorescence ratio and pluripotency characterization.
A: The trace showed the protocol of BCECF fluorescence ratio (510 nm emission at 490 nm and 440 nm excitations) calibration in HPS0077 cells. The top bars represent the application of different conditions; B: The plots of pHivs the BCECF fluorescence ratio were collected from 6 similar experiments shown in A; C: Immunofluorescence analysis showed the expression of pluripotency markers, OCT4, SSEA4, TRA-1-60 and SOX2, in HPS0077 cells.
- Citation: Chao SC, Wu GJ, Huang SF, Dai NT, Huang HK, Chou MF, Tsai YT, Lee SP, Loh SH. Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells. World J Stem Cells 2018; 10(12): 196-211
- URL: https://www.wjgnet.com/1948-0210/full/v10/i12/196.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v10.i12.196