幽门螺杆菌重组VacA-HpaA IgY的制备

图1 重组蛋白表达形式的鉴定. 1: protein marker; 2,3: pQE30-v/h-DH5a induced without IPTG; 4,5: supernatant of pQE30-v/h-DH5α induced with IPTG; 6,7: precipitation of pQE30-v/h-DH5α induced with IPTG.

图2 纯化的重组VacA-HpaA的SDS-PAGE分析. M_r: protein marker; 1-4: purified recombinant protein (E1, E2, E3, E4).

图3 免疫时间与抗体效价变化. PID: 第1次免疫后的时间.

图4 纯化浓缩后IgY的SDS-PAGE分析. 1: protein marker; 2-5: purified recombinant protein.

图5 VacA-HpaA IgY的Western blot鉴定. 1,4: protein marker; 3,6: control(without induction); 2: VacA-HpaA IgY responsed with VacA; 5: VacA-HpaA IgY responsed with HpaA.