Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene

doi:10.3748/wjg.v9.i9.1892

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Sep 15, 2003 (publication date) through Apr 30, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Esophageal Cancer

World J Gastroenterol. Sep 15, 2003; 9(9): 1892-1896
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.1892

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Figure 2 Positive colonies screened by yeast two-hybrid sys-tem using full-length cDNA of ECRG2 as baits. A: PCR products of positive clones digested with HaeIII restriction enzyme. Lane 1: λDNA/EcoR I+Hind III Marker, Lanes 2-16: positive colonies, B: Trp⁺/Leu⁺/His⁺/Ade⁺ positive clone growing on the SD/Trp^-/Leu^-/His^-/Ade^- plate, C: colony-lift filter assay for β-galactosidase activity.

Citation: Cui YP, Wang JB, Zhang XY, Bi MX, Guo LP, Lu SH. Using yeast two-hybrid system to identify ECRG2 associated proteins and their possible interactions with ECRG2 gene. World J Gastroenterol 2003; 9(9): 1892-1896
URL: https://www.wjgnet.com/1007-9327/full/v9/i9/1892.htm
DOI: https://dx.doi.org/10.3748/wjg.v9.i9.1892