Contribution of eIF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line: LS-174T

Figure 4 Effects of eIF-4E down-regulation upon enzyme activity of heparanase. Cells were treated for 48 h with antisense oligonucleotide (■), sense oligonucleotide (▲) or liposome only(◆). The soluble fraction from transfected, freezed and thawed LS-174T cells was incubated with ³⁵S-la-beled HS. The incubation medium was then subjected to gel filtration over Sepharose CL-6B. Low-molecular-weight HS degradation fragments (peak II) were mainly produced dur-ing incubation with medium conditioned by sense oligonucle-otide infected cells. The cells treated with antisense oligonucle-otide exhibited low levels of heparanase activity as compared to untreated ones. Heparanase activity was also expressed as Kav×total cpm eluted in peak II.