Contribution of eIF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line: LS-174T

Figure 3 Western blot analysis of heparanase expression in LS-174T cells. The colon adenocarcinoma cells were lysed, and Western blot analysis was performed with an anti-hu-man heparanase polyclonal antibody. AS, antisense oligo-nucleotide-treated cells; S. sense oligonucleotide-treated cells; C, cells treated with liposome only. Cells treated with anti-sense oligonucleotides showed markedly reduced levels of heparanase protein compared to cells treated with sense oli-gonucleotides or saline in the presence of liposome. The ap-proximately 65-kDa protein represents a heparanase precursor, whereas the 50-kDa protein is a processed form, and data showed that both of them were less expressed while eIF-4E was inhibited.