Contribution of eIF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line: LS-174T

Figure 2 eIF-4E protein expression in oligonucleotide-treated colon adenocarcinoma cells. Protein extracts were prepared from oligonucleotide-treated cells at 48 h after transfection. Cell lysates were size-fractionated by 12% SDS-PAGE, and immunodetection on the blotted membrane was performed using the eIF-4E MAb. AS, antisense oligonucle-otide-treated cells; S. sense oligonucleotide-treated cells; C, cells treated with liposome only; PC, eIF-4E standard anti-gen (derived from a human neuroblastoma cell line) as a positive control marker.