Analysis of spontaneous, gamma ray- and ethylnitrosourea-induced hprt mutants in HL-60 cells with multiplex PCR

Table 1 Oligonucleotide primers in multiplex PCR of the hu-man HPRT locus

Exons	Primers sequence (5’-3’)	Fragment size (bp)
1	F TGG GAC GTC TGG TCC AAG GAT TCA	626
	R CCG AAC CCG GGA AAC TGG CCG CCC
2	F CCT GAT ATG CTC TCA TTG AAA CA	211
	R GCT GCT GAT GTT TGA AAT TAA CAC
3	F GTT TAA TGA CTA AGA GGT GTT TG	311
	R GAA AAC CTA GTG TTG CCA CAT AA
4	F GTG TGT GTA CAT AAG GAT ATA CA	165
	R TTC TTC CCT TTC AAG ATA CAT AC
5	F GGA AAT ACC GTT TTA TTC ATT GT	125
	R GTG CAT ACT AAG TTA GAA AGG TC
6	F GTG ACT CTG AAT TTA AAG CTA TG	150
	R CTG TGT CAA AAT GTC ATA CAT AC
7/8	F GTC TCT CTG TAT GTT ATA TGT CAC	379
	R TGC GTG TTT TGA AAA ATG AGT GAG
9	F GCT ATT CTT GCC TTT CAT TTC AG	136
	R CAA ACT CAA CTT GAA CTC TCA TC