Establishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization

Figure 5 IL-1β activated NF-κB and AP-1 in SIPS cells. (A, B) SIPS cells were treated with IL-1β (at 2 ng/ml, lane 2) for 1 hour. Nuclear extracts were prepared, and specific binding activities of NF-κB (panel A) and AP-1 (panel B) were assessed by electro-phoretic mobility shift assay. Arrows denote specific inducible complexes competitive with cold double-stranded oligonucle-otide probes. Lane 1: control (medium only). a: non-specific band. (C) SIPS cells were treated with IL-1β for indicated times. Total cell lysates (approximately 100 μg) were prepared, and the level of IκB-α was determined by Western blotting.