Establishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization

Figure 2 SIPS cells were negative for SA-β-Gal. SIPS cells (A), SAM-K cells (B), and late-passage (passage 20) PSCs (C) were grown directly on slides, fixed, and stained with β-Gal staining solution, adjusted to pH 6.0, for 16 hours at 37 °C. After washed, the slides were viewed under light microscope. SA-β-Gal was not detected in SIPS and in SAM-K cells, but was detected in late-passage PSCs (arrows). Original magnification: ×20 objective.