Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma

Table 1 Primer sequences for the coding region of the LPTS gene

Exon	Nucleotide sequences	PCR conditions	Product size (bp)
1	Forward: 5’-CGTGCTCGAGGAGCGAGTCG-3’
	Reverse: 5’-ACCCGGCATCTTCACCAACG-3’	52 °C SD	241
2	Forward: 5’-TCCATTGCTGATGATAATGC-3’
	Reverse: 5’-CTTCCAGTCTCCTAAGAAGG-3’	50 °C SD	230
3	Forward: 5’-TGAGAGGAATGTTCTAACTC-3’
	Reverse: 5’-AGCCAAYYAYGCAAAGACAC-3’	50 °C SD	178
4	Forward: 5’-AACTACAGGCTTACCTCTCG-3’
	Reverse: 5’-AACATATTTGCATTGAGAAC-3’	55 °C SD	196
5	Forward: 5’-CAAGACTATCCACTGTTAGG-3’
	Reverse:5’-GGACAAACACGTAGATTTCAATAAC-3’	52 °C Standard	220
6	Forward: 5’-GCTGCATAGTTCATGTCTGC-3’
	Reverse: 5’-CACAGGTGAAAATCAGACAG-3’		194
7.1	Forward: 5’-CTGCCTTTTAACTCTTCTGC-3’
	Reverse: 5’-GGCTGGAGGTAACTTTCCAC-3’	50 °C SD	247
7.2	Forward: 5’-GGCCACAGGTAAAGATGTGG-3’
	Reverse: 5’-CAGGCGGCTGCACATGGTCC-3’	55 °C Standard	200
7.3	Forward: 5’-GCCTCTGCTCAGGATGCAGG-3’
	Reverse: 5’-GCCCCGGCTGGGAAGGATTC-3’	50 °C SD	191

SD: step-down PCR