Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells

Figure 1 Detection of expression of VEGF and VEGFR in eight gastric carcinoma cell lines by RT-PCR. A. VEGF was ampli-fied using primers designed to detect all known splicing vari-ants and two isoforms 531 bp and 663 bp corresponding to VEGF₁₂₁ and VEGF₁₆₅ were obtained in all cell lines. B. Flt-1 of the expected size (1212 bp) was amplified in all cell lines except KATOIII. C. KDR of the expected size (927 bp) was amplified in all cell lines except SNU-5 and KATOIII. D. GAPDH was amplified in each cell line as a positive control for RT-PCR.