Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation

Table 1 The primers used for amplification of HAV genomic RNA

Cloned fragments	Primers	Sequences
A (0.8 kb)	A1	5'-CGCCGGCGTTCAAGAGGGGTGTCCGGAG-3'
	A2	5'-GAATCTCAATGCCAAATCTTGC-3'
B (0.5 kb)	B1	5'-TCTGAGGTACTCAGGGGC-3'
	B2	5'-CAGTCAATGATGCTATAGAACC-3'
C (1.1 kb)	C1	5'-CCAACAGGGGGGATTGATC-3'
	C2	5'-CGTTAGAAGGAGAGGTCAATC-3'
D (1.0 kb)	D1	5'-CCCTGGATTTCTGACACTCC-3'
	D2	5'-CAGTGGATAACATGGCATTTG-3'
E (1.1 kb)	E1	5'-GTCTGTCACAGAACAATCAGAG-3'
	E2	5'-GATCCCAGAACAGATATCTCTTAA-3'
F (1.2 kb)	F1	5'-GTTAAGAGATATCTGTTCTGGATC-3'
	F2	5'-CCATCCTCCAACGAGCACTCC-3'
G (1.2 kb)	G1	5'-CAGTTCTTTAGTCATGACAGTTG-3'
	G2	5'-GCCATTGGATCAATTTCAGC-3'
H (1.1 kb)	H1	5'-GAGTCCCATTTATCATCACA-3'
	H2	5'-GTCCAATCAGATCAAGATTATC-3'
I (0.5 kb)	I1	5'-GATTCTCTGTTATGGAGATG-3'
	I2	5'-TTTTTTTTTTTTTTTTTTTTTATTT-3'

⁽¹⁾: A-I represent 9 amplified fragments of HAV; ⁽²⁾ 1 and 2 represent positively and negatively oriented primers