Changes of NF-κB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species

Figure 7 P53, bcl-2 protein levels in AGS cells treated with JTE-522. Cell lysates were collected and processed at 6 h. The whole cellular protein was electrophoresed in SDS-PAGE gel, Western blot was performed using antibodies against p53, bcl-2. Beta actin was used as a lane-loading control. (1) control; (2) 0.25 mmol/L; (3) 0.5 mmol/L; (4) 0.75 mmol/L; (5) 1 mmol/L. Representative results from three independent experiments.