Changes of NF-κB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species

Figure 6 Effect of JTE-522 on NF-κB binding activity and IkBα degradation. Cells were treated with JTE-522 for 6 h. Cells were harvested and EMSA was performed as described (A). Lane 1: control; lane 2-5: AGS cells treated with 0.25, 0.50, 0.75, 1 mmol/L of JTE-522. The identity of DNA-complexed pro-teins was confirmed by supershift assays using antibodies against p65 sub-unit of NF-κB (lane 6). Immunobolt analysis of IkBα of corresponding cytosilic supernatant (B). Representative results from four independent experiments.