Rapid screening mitochondrial DNA mutation by using denaturing high-performance liquid chromatography

Figure 2 The universality of a broad small peak proceeding the main peak induced by Taq DNA polymerase. An extra small peak is observed preceding the eluted chromatography profile of A and B but not in chromatogram of C, D and E. Chromatogram A is a representative of products amplified by Taq DNA polymerase. B is typical chromatography profile when Taq DNA polymerase matched with Pfu DNA polymerase buffer in PCR reaction. C and D respectively comes from amplimers amplified by Ampli-Taq Gold and Vent DNA polymerase. E is one of chromatograms coming from PCR amplification by using Pfu DNA polymerase.