Inhibitory effect of endostatin expressed by human liver carcinoma SMMC7721 on endothelial cell proliferation in vitro

Figure 5 Inhibition of endothelial cell proliferation by conditioned medium from transfected and untransfected cells. Conditioned medium from endostatin-transfected SMMC-Endo cells (2), conditioned medium from SMMC7721 cells (3), and conditioned medium from SMMC-pLncx cells (4) were concentrated and applied to cultivate with HUVEC cells grown in 40-well plate. Three days later, cell number, as measured by absorbance (OD), was then quantified by using a colorimetric MTT assay. Bars, SD. ^bP < 0.01, compared with conditioned medium from control SMMC-pLncx cells.