Copper transportion of WD protein in hepatocytes from Wilson disease patients in vitro

Table 1 Concentrations of copper in organelles of nor mal hepatocytes after 2 h or 24 h culture (mean ± SD, μg•g¯¹)

t (culture)/h	ATP/(30 mmol•L¯¹)	Microsomes	Lysosomes	Cytoplasm	Mitochondria
0		74 ± 13	70 ± 10	526 ± 63	85 ± 11
2	absence	231 ± 31	306 ± 19	1571 ± 115	420 ± 43
2	presence	269 ± 43a	342 ± 26a	1488 ± 129a	395 ± 35
24	absence	346 ± 52	322 ± 40	1589 ± 137	458 ± 68
24	presence	288 ± 39a	369 ± 46	1464 ± 110b	417 ± 73a

^aP < 0.05, vs ATP absence;

^bP < 0.01, vs ATP absence.