Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

Table 1 Lung metastases of 10 clones

Clone number	n Tumor implanted	n Lung metastases after 5 wk
1, 7, 8, 10, 11, 13	6	6
4	6	5
6, 24	6	4
25	6	2
MHCC97	6	6

NOTE. Each clone was first sc inoculated into nude mice. Then small pieces of subcutaneous tumor were implanted into the liver of 6 nude mice for every clone. The number of mice with lung metastases was determined by histopathology 5 wk after orthotopic tumor implantation (see the in vivo screening for detail.).