Original Articles
Copyright ©The Author(s) 2001.
World J Gastroenterol. Jun 15, 2001; 7(3): 324-330
Published online Jun 15, 2001. doi: 10.3748/wjg.v7.i3.324
Figure 7
Figure 7 Detection of human albumin mRNA in rat livers by RT-PCR. Panel A, RT-PCR of RNA extracts from liver samples collected at wk16 post-transplantation. Lane 1, 1 kb plus molecular markers; lanes 2 and 6, non-intrafetally injected, non-transplanted rats; lanes 3 and 7, Huh7 cells as positive control; lanes 4 and 8, rats intrafetally injected, and transplanted with primary human hepatocytes; lanes 5 and 9, rats intrafetally injected, but not transplanted with human hepatocytes. For lanes 2 to 5, samples were amplified with primers for human albumin DNA, and for lanes 6 to 9, samples were amplified with primers for rat albumin DNA. In lanes 10 to 12, DNA from 104, 103 and 102 cultured Huh7 cells were amplified with primers for human albumin. The expected positions of human and rat albumin mRNA products at 315 bp and 388 bp, respectively, are indicated by arrows. Panel B, Time course of human albumin mRNA expression in the rat livers. Lane 1, 1 kb plus molecular markers; lane 2, samples from a non-tolerized and non-transplanted rat; lane 3, Huh 7 cells as a positive control; lanes 4 to 6, samples from a representative rat tolerized and transplanted with primary human hepatocytes collected at wk2, 6 and 16 post-transplantation, respectively.