Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice

Table 1 Specific inhibition of SMMC-7721 human hepatoma cell growth by alpha-fetoprotein (AFP) antisense phosphorothioate oligodeoxyribonucleotides (S-ODNs) in vitro

Concentration (μmol/L)	Hepatoma cell growth (absorbance at 570 nm)
	Sense	Antisense	Oligo dT15
0	0.83 ± 0.10	0.83 ± 0.10	0.83 ± 0.10
6.3	0.84 ± 0.08	0.50 ± 0.08 (39.8)a	0.82 ± 0.11
12.5	0.76 ± 0.06	0.44 ± 0.04 (46.9)a	0.87 ± 0.07
25.0	0.72 ± 0.13	0.42 ± 0.03 (49.4)a	0.88 ± 0.18

SMMC-7721 cells were incubated with various concentrations of S-ODNs for 48 h in vitro. A specific growth inhibition was observed by the treatment with antisense S-ODNs. Values in parentheses: percent inhibition. Each value represents mean ± SD of 3 separate experiments.

^aP < 0.05 (Student's t test) compared with 0 μmol/L.