Characterization of six tumor suppressor genes and microsatellite instability in hepatocellular carcinoma in southern African blacks

Table 1 PCR primers

Gene/Locus	Primer	Primer sequence	Amplicon	Amplicon length
p53	p53F3	5’GTTGGCTCTGACTGT-ACCAC	exon 7 spanning codon 249[6]	110 bp
	p53R3	5’CTGGAGTCTTCCAGT-GTGAT
p53	p53ivs1a	5’GCACTTTCCTCAACTCTACA	ALU sequence within intron 1	200 bp-300 bp
	p53ivs1b	5’AACAGCTCCTTTAATGGCAG	of p53 gene[43]
D13S120	1353L	5’ATGACCTAGAAATGATACTGGC	(AC)73 repeat at D13S120[44]	112 bp-136 bp
(BRCA2)	1353R	5’CAGACACCACAACACACATT
D17S846	FF	5’TGCATACCTGTACTACTTCAG	(GGAA)25 repeat at D17S846[45]	250 bp-300 bp
(BRCA1)	RF	5’TCCTTTGTTGCAGATTTCTTC
D17S855	FS	5’GGATGGCCTTTTAGAAAGTGG	AC repeat at D17S855[46]	145 bp
(BRCA1)	RS	5’ACACAGACTTGTCCTACTGCC
WT1	400	5’AATGAGACTTACTGGGTGAGG	AC repeat within 3’ untranslated	100 bp-200 bp
	401	5’TTACACAGTAATTTCAAGCAACGG	sequence of WT33[47]
RB1	B57	5’TGTATCGGCTAGCCTATCTC	[CTTT(T)]n (n = 14-26) repeat	400 bp-600 bp
	B103	5’AATTAACAAGGTGTGGTGGT	within intron 20 of RB gene[48,49]
D13S127	1341L	5’CAGATATGTACTCATGCACATG	(AC)35 repeat at D13S127[44]	130 bp-142 bp
(BRCA2/RB1)	1341R	5’AAACAAATGAGTTTGGCTGT
D13S137	F	5’TTTCCTCATTCTTTCCCAATTG	(GT)22 repeat at D13S137[50]	± 135 bp
(RB1)	R	5’CAGGAGGGATGGACTCACTTC
E-cadherin	E-cadF1	5’GATCCTAAGGACAAATGTAGATGCTCT	D16S301 locus polymorphic	146 bp
	E-cadF1	5’AGCCACTTCCCAGAACTTGGCTTCC	AC region[51]
E-cadherin	E-cadF2	5’GGTTGAGATGCTGACATGC	D16S260 locus polymorphic	± 234 bp
	E-cadR2	5’CAGGGTGGCTGTTATAATG	AC repeat region[52]

Note: WT1: Wilm’s tumor gene; RB1: Retinoblastoma gene; BRCA1: Brea st cancer susceptibility gene 1; BRCA2: Breast cancer susceptibility gene 2; bp: base pairs.