HBeAg gene expression with baculovirus vector in silk worm cells

Table 2 Comparison of two methods in anti-HBe antibody detection

Conventional method	n	Positive (n)
		Double sandwich methoda	Indirectb
Anti-HBe(+) and anti-HBc(-)	35	35	35
Anti-HBe(-) and anti-HBc(+)	52	4	52
Anti-HBe(+) and anti-HBc(+)	37	37	37
Anti-HBe(-) and anti-HBc(-)	42	2	0

^aThe purified HBeAg expressed in karyotic cells was used to coat enzyme-labeled reaction plate (100 ng/well), incubated throughout the night at 4 °C, and then serum to be detected was added to it. After 30 min at 40 °C, HRP-Labeled HBeAg expressed in karyotic cells was added to it, and at last this was colorized by application of TMB-H₂O₂. Those P/N ≥ 2.1 were positive.

^bThe anti-HBe antibody detection kit for sale in market employed the competition inhibition method.